Research Paper Volume 9, Issue 3 pp 706—740

Genomic deletion of GIT2 induces a premature age-related thymic dysfunction and systemic immune system disruption

Figure 7. GIT2 genomic deletion engenders a consistent transcriptomic signature across multiple immune tissues. Venn diagram analysis of the significant transcriptomic effects of GIT2 deletion in the ILN, MLN, spleen and thymus of GIT2KO mice compared to age-matched WT controls. In the Venn diagram numbers in italics represent upregulated transcripts, underlined numbers represent downregulated transcripts, red numbers represent transcripts possessing diverse expression polarities (A). Hierarchical wordcloud generated using the collective processing mode of Textrous! to investigate the functional nature of the 40 coherently-regulated transcripts common across GIT2KO ILN, MLN, spleen and thymus (B). Physical proximity of semantically-associated scientific words in public biomedical database curated documents indicates their strength of relationship. The most strongly associated words (with the entire input 40 transcript dataset) occur in the more intense red-hued regions of the cloud. A cumulative z ratio representation of the 40 coherently-regulated cross-tissue (ILN – black bars; MLN – red bars; spleen – green bars; thymus – blue bars) GIT2KO-spepcific factors indicates the strong presence of pro-aging/stress phenotype that is closely linked with clock gene dysfunction (stress/clock gene related transcripts possess Gene Symbols in bold typeface) (C).