Microvesicles from the plasma of elderly subjects and from senescent endothelial cells promote vascular calcification

Matilde Alique; María Piedad Ruíz-Torres; Guillermo Bodega; María Victoria Noci; Nuria Troyano; Lourdes Bohórquez; Carlos Luna; Rafael Luque; Andrés Carmona; Julia Carracedo; Rafael Ramírez

doi:doi:10.18632/aging.101191

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Research Paper Volume 9, Issue 3 pp 778—789

Microvesicles from the plasma of elderly subjects and from senescent endothelial cells promote vascular calcification

Figure 2. HUVEC-derived MVs assessed by flow cytometry. Representative dot plots showing log forward scatter (FSC) vs. log side scatter (SSC) from young (A1) and senescent (B1) HUVEC. Representative dot plots showing annexin A5 and CD31 phenotype from young (A2) and senescent (B2) HUVEC. (C) Absolute number of MVs per µL of sample from young and senescent HUVEC; *p<0.05. (D) Absolute number of CD31+/annexin A5+ MVs per µL of sample; *p<0.05. Results are presented as the mean ± SD of 6 independent experiments.