Research Paper Volume 9, Issue 3 pp 778—789

Microvesicles from the plasma of elderly subjects and from senescent endothelial cells promote vascular calcification

Figure 3. MVs from senescent HUVEC cells induced calcification in HASMC. Cells were cultured with 50,000 MVs/mL from young (Y MV) or senescent (S MV) HUVEC for different times. (A) Qualitative calcification was stained with alizarin red. A representative experiment from six different experiments per duplicate is show. (B) Calcium content was determined by spectrophotometer by phenolsulphonephthalein dye at 6 and 9 days of treatment. The graphs present calcium content of the cells expressed as µg/mg protein. The data represent means ± SD of 3 independent experiments. *** p<0.001 vs control and Y MV at the same time.