Research Paper Volume 9, Issue 3 pp 803—822

Age-associated chromatin relaxation is enhanced in Huntington’s disease mice

Figure 3. Increased expression of Polycomb repressive complex (PRC)-regulated genes (PRG) in aged mouse models of Huntington’s disease (HD). (A) The proportion of amplicons in each category that belong to the upper (red) and lower (black) clusters in the heat map shown in Figure 2A. The upper cluster amplicons represent 26% of the total amplicons as indicated by dotted line (red). TSG, tumor suppressor genes. For other abbreviations, refer to the Figure 2 legend. (B-E) Fold difference (log2) in the amount of PRG amplicons between aged HD vs. young HD (B), between aged HD vs. aged wild-type (wt) (C), between aged wt and young wt (D), and between young HD vs. young wt samples (E). P-values, paired-sample t-test. Error bar, standard error. (F) Comparison of expression levels of Ezh2 in HD and wild type samples. P-value, paired-sample t-test. Error bar, standard deviation. (G) RT-PCR analysis of PRGs. PRGs were randomly chosen, and expression levels were examined in splenic T cells from young (HD_y) and aged HD (HD_o) mice. 18s RNA, loading control.