Research Paper Volume 9, Issue 3 pp 932—954

MiR-34a-3p alters proliferation and apoptosis of meningioma cells in vitro and is directly targeting SMAD4, FRAT1 and BCL2

Figure 1. Dual luciferase reporter gene assays for SMAD4, FRAT1 and BCL2. (A, B, C) Schematic representation of luciferase reporter gene constructs with wild-type miRNA binding sites (3’UTR) and mutated variants (3’UTR mut) for SMAD4, FRAT1 and BCL2. (D, E, F) Dual luciferase reporter gene assays 48 h after co-transfection of HEK293T cells with reporter gene constructs (pMIR-RNL-TK) with the respective wild-type or mutated 3‘UTR for each target and control (pSG5) or miRNA-expression construct (pSG5-miR-34a) in the indicated combinations. Relative luciferase units (RLU) are means ± SEM of three independent experiments performed in duplicates (*, P < 0.05; **, P < 0.01; ***, P < 0.001).