Figure 1. Mitochondrial lipid composition differs between tissue types and changes with age. (A) Orthogonal partial least square-discriminant analysis (OPLS-DA) of lipids found in the mitochondria of murine brain and skeletal muscle. Separation across the x-axis is according to tissue type. Along the y-axis separation delineates age. There are clear lipid compositional alterations between the mitochondria isolated from the two tissue types and with differences in age of the tissue. (B) OPLS-DA of lipid composition of murine brain mitochondria aged 4-11 weeks (pink) (n=10) and 78 weeks (red) (n=10). (C) OPLS-DA of the lipid composition of murine muscle mitochondria aged 4-11 weeks (yellow) (n=9) and 78 weeks (blue) (n=10).