Figure 5. LOC100129148 is a direct target of miR-539-5p. (A-B) Detection of targeted miRNAs using qRT-PCR in the sample pulled down by biotinylated FTH1P3 probe. (C) Sequence alignment of miR-539-5p with the putative binding sites within the wild-type regions of LOC100129148. (D-E) The luciferase report assay demonstrated that overexpression of miR-539-5p could reduce the intensity of fluorescence in CNE-1 and SUNE-1 cells transfected with the LOC100129148-WT vector, while had no effect on the LOC100129148-MUT vector. Assays were performed in triplicate. *P< 0.05. Means ± SEM are shown. Statistical analysis was conducted using student t-test.