Priority Research Paper Volume 9, Issue 3 pp 615—626

Physiological frailty index (PFI): quantitative in-life estimate of individual biological age in mice

Figure 3. Sex-specific effects of rapamycin on lifespan and health of NIH Swiss mice. (A) Animals receiving rapamycin in drinking water maintain their body weights comparable to control mice. Blue line – normal drinking water; red line – water with rapamycin. (B) Kaplan-Meier survival curves for mice fed regular chow in combination with normal drinking water (blue line) or rapamycin (red line). Chronic administration of rapamycin has no effect on longevity of male mice (mean survival is 113.91+6.98 and 100.8+6.96 weeks for control and rapamycin-treated mice respectively. In females, rapamycin administration increases lifespan from 110.09+7.12 to 131.23+8.29 weeks (p=0.05, Kaplan-Meier log-rank test). Red arrow indicates the period of time when mice received rapamycin. Black arrow indicates time when PFI was measured. (C) PFI created at described above. No effect of rapamycin on health status was detected in male and female mice fed regular chow. RDW – regular diet in combination with normal drinking water (group1); RDR – regular diet in combination with rapamycin (group 2).