Figure 2. Validation of aging associated miRNA expression profile. (A and B) A portion of RNA used in the microarray was used in qPCR (A) and in a separate experiment by solution hybridization technique with 5’ biotin-labeled miRNAs (B) to confirm the expression level of miRNAs that were differentially regulated ≥1.5-fold in the microarray. U6 served as both loading control and normalizer. Gel pictures are representative of three independent experiments. Each bar indicates mean ± SEM (n = 3). White and black bars indicate the levels of miRNA measured by microarray and qPCR, respectively.