Priority Research Paper Volume 9, Issue 3 pp 627—649

Mitochondrial protein Fus1/Tusc2 in premature aging and age-related pathologies: critical roles of calcium and energy homeostasis

Figure 6. Fus1 loss results in a decreased maximal respiration and respiratory reserve capacity in primary MEFs and immortalized epithelial Fus1 KO cells. High resolution respirometry oxygraph-2k (Oroboros Instruments) was used for analysis of cellular respiration. Abbreviations are as follows: OCR - oxygen consumption rate, BR - basal respiration rate, PL - proton leak rate, ALR - ATP-linked respiration; MR - maximal mitochondrial respiration, RRC – respiratory reserve capacity, NMR - non-mitochondrial respiration. Experiments were performed in triplicates. *p-value ≤ 0.05; **p-value ≤ 0.005 (Student's t-test, 2-sided unpaired). Data expressed as mean ± SEM.