Figure 1. Functional Sirt6 is expressed in mouse kidney. (A) Body weight of Sirt6 KO mice and their WT littermates was quantified at various ages. Data are presented as mean ± SEM; n=8-9; *P<0.05, ***P < 0.001 compared with relevant WT mice; #P<0.05 compared with 1 month old WT mice. (B) Nuclear protein was extracted from the kidneys of WT and Sirt6 KO mice and Sirt6 protein expression was assessed by Western blot analysis. Lamin B1 served as loading control. (C) Immunofluorescence of Sirt6 and H3K56 in kidney cryosections from WT and Sirt6 KO mice.