Figure 2. Sirt6 deficiency causes kidney hypertrophy and proteinuria. (A, B) Kidneys from WT and Sirt6 KO mice were collected at 1, 2, 7 months of age. The weight of kidney was quantified (A) and the ratio of kidney weight to body weight was calculated (B). (C) Representative image of the kidneys from WT and Sirt6 KO mice at 7 months of age. (D) Periodic acid–Schiff staining of kidney paraffin section for histologic analysis. (E) Quantification of glomerular area (~15 glomeruli/section). (F) Urine was collected from WT and Sirt6 KO mice at indicated time points. Albumin and creatinine were measured by Albumin ELISA kit and Creatinine Colorimetric Assay Kit respectively, and ACR was calculated. Data are presented as mean ± SEM; n=6-9; **P<0.01, ***P <0.001 compared with relevant WT mice; #P<0.05 compared with 1 month old WT mice.