Research Paper Volume 9, Issue 3 pp 1069—1083

Sirt6 deficiency results in progression of glomerular injury in the kidney

Figure 3. Sirt6 deletion induces podocyte injury. (A-C) Immunostaining of podocin and nephrin (green) in the kidney cryosection from WT and Sirt6 KO mice at different ages. (D, E) Graphs represent the quantification of podocin and nephrin immunofluorescence. Data are presented as mean ± SEM. **P<0.01, ***P<0.001 compared with relevant WT mice. (F) The cortex of kidney from WT and Sirt6 KO mice was collected at 7 months of age and lysed, and podocyte slit diaphragm proteins (podocin and nephrin) and podocyte injury marker desmin were examined by Western blot analysis. (G) Graph represents the densitometry of podocin, nephrin and desmin expression normalized to β-actin. Data are presented as mean ± SEM; n=3; *p<0.05 compared WT.