Figure 4. Sirt6 deletion results in podocyte foot process effacement and cellular loss. (A) Ultrastructural analysis of the kidneys from WT and Sirt6 KO mice at 2 months of age by transmission electronic microscopy. P: podocyte; cap: capillary. (B) Kidney cyrosections from WT and Sirt6 KO mice at 2 and 7 months of age were stained with antibody against podocyte marker WT-1 (red) and images were taken by fluorescence microscopy. Blue: DAPI staining for nucleus. Podocyte numbers were counted randomly in 15 glomeruli/section and quantified. Data are presented as mean ± SEM. ***p<0.001 compared with 7-month old WT mice.