Research Paper Volume 9, Issue 3 pp 1069—1083

Sirt6 deficiency results in progression of glomerular injury in the kidney

Figure 4. Sirt6 deletion results in podocyte foot process effacement and cellular loss. (A) Ultrastructural analysis of the kidneys from WT and Sirt6 KO mice at 2 months of age by transmission electronic microscopy. P: podocyte; cap: capillary. (B) Kidney cyrosections from WT and Sirt6 KO mice at 2 and 7 months of age were stained with antibody against podocyte marker WT-1 (red) and images were taken by fluorescence microscopy. Blue: DAPI staining for nucleus. Podocyte numbers were counted randomly in 15 glomeruli/section and quantified. Data are presented as mean ± SEM. ***p<0.001 compared with 7-month old WT mice.