Figure 5. Sirt6 knockdown impairs foot process and induces podocyte apoptosis in vitro. Primary murine podocytes were plated in a collagen coated 12-well plate at a density of 6x104 cells/well and transfected with Sirt6 or control siRNA. (A) Two days after transfection, images of cell morphology change were taken by light microscope. (B, C) Three days after transfection, cells were harvested for western blot (B) and Cell death ELISA (C). Data are presented as mean ± SEM; n=3; *p<0.05 compared control siRNA.