Figure 6. Sirt6 deficiency increases inflammation in the kidney. (A) The cortex of kidney from WT and Sirt6 KO mice was collected at indicated time points and mRNA was extracted. The expression of Cxcl10, Nox2, Tweak and Fn14 mRNA was assessed by quantitative PCR. Data are presented as mean ± SEM; n=6; *p<0.05; **P<0.01; ***p<0.001 compared with relevant WT mice. (B-D) Kidney cyrosections from WT and Sirt6 KO mice at different ages were stained for leucocyte subtype markers CD45, Iba1, CD3 and Ly6G (green). Images were taken at 200X by fluorescence microscopy.