Research Paper Volume 9, Issue 4 pp 1153—1185

The age- and sex-specific decline of the 20s proteasome and the Nrf2/CncC signal transduction pathway in adaption and resistance to oxidative stress in Drosophila melanogaster

Figure 10. Decline in 20S proteasome induction is accompanied by an accumulation of oxidized proteins. Carbonyl content was detected with a DNP antibody in progeny of the Actin-GS-255B strain crossed to w[1118] strain following [0, 10, or 100µM] H2O2 pre-treatment for 8 hours, followed by a 16-hour recovery to allow for adaption before challenged with H2O2 [4.4M] for an additional 24 hours. (A) Carbonyl content showed significant decrease following H2O2 pretreatment in 3 day old females. (B) Carbonyl content showed no significant change in 60 day old females, irrespective H2O2 pretreatment. (C) Carbonyl content was measured in 3 day old males that were pre-treated with H2O2. (D) 60 day old males showed no change in carbonyl content upon H2O2 pre-treatment and subsequent recovery. Western blots were performed in triplicate and carbonyl content was normalized to Actin-HRP. Error bars denote standard error of the mean (S.E.M) values. * P <0.05, ** P <0.01, *** P < 0.001, relative to the young control using one-way ANOVA. Statistical significance is shown with asterisks (pink *) in young females.