Figure 10. Decline in 20S proteasome induction is accompanied by an accumulation of oxidized proteins. Carbonyl content was detected with a DNP antibody in progeny of the Actin-GS-255B strain crossed to w strain following [0, 10, or 100µM] H2O2 pre-treatment for 8 hours, followed by a 16-hour recovery to allow for adaption before challenged with H2O2 [4.4M] for an additional 24 hours. (A) Carbonyl content showed significant decrease following H2O2 pretreatment in 3 day old females. (B) Carbonyl content showed no significant change in 60 day old females, irrespective H2O2 pretreatment. (C) Carbonyl content was measured in 3 day old males that were pre-treated with H2O2. (D) 60 day old males showed no change in carbonyl content upon H2O2 pre-treatment and subsequent recovery. Western blots were performed in triplicate and carbonyl content was normalized to Actin-HRP. Error bars denote standard error of the mean (S.E.M) values. * P <0.05, ** P <0.01, *** P < 0.001, relative to the young control using one-way ANOVA. Statistical significance is shown with asterisks (pink *) in young females.