Figure 2. Adaptation to hydrogen peroxide declines with age in females. Progeny of the Actin-GS-255B strain crossed to the w strain were aged to 3 days and 60 days prior to H2O2 pretreatment. Following recovery, flies were fed H2O2 challenge dose: 4.4M in 3 day old males and females, 2M in 60 day old females, and 1M in 60 day old males. (A) 3 day old females showed increased survival following pretreatment. (B) 3 day old males showed no change in survival following pretreatment. (C) With age, 60 day old females no longer show increased survival. (D) 60 day old males show no change in adaptation following pretreatment. Statistical difference in survival (p < 0.05) was calculated using the Log-Rank test. Statistical summary is located in Supplementary Table S1.