Research Paper Volume 9, Issue 4 pp 1219—1232

The differential spatiotemporal expression pattern of shelterin genes throughout lifespan

Figure 1. Shelterin components are differentially expressed during development and adulthood. Quantitative RT-PCRs for Shelterin components Trf1, Trf2, Rap1, Tpp1, Tin2, Pot1a, and Pot1b and PCNA as a marker for proliferation in mouse brains, hearts, livers, and kidneys at different time-points of development and in adulthood (n=4 each, the four samples for E10 were each pooled from 7 organs, at E12 and 14 the four samples were pooled from four organs each). Expression of each gene was normalized to the respective Gapdh, actin, and Rplp0 expression. Next, the average of all organs and samples at E10.5 was calculated. Individual samples were then normalized against this average value. Significance was tested between E10 and P100 (adult). Data are mean ± SEM. *p<0.05, **p<0.01, ***p<0.001.