Research Paper Volume 9, Issue 4 pp 1233—1247

Aspirin inhibits the SHH/GLI1 signaling pathway and sensitizes malignant glioma cells to temozolomide therapy

Figure 1. Effects of aspirin on glioma cells survival and proliferation. (A) U87 and T98G cells were treated with increasing concentrations of aspirin for 24 h. Cell survival was analyzed by CCK-8 assay. (B) U87 and T98G cells were treated with indicated concentrations of aspirin and harvested after 24, 48 and 72 h. Cell proliferation was analyzed by CCK-8 assay. (C) The glioma cells viability upon indicated concentrations of aspirin treatment were determined by colony formation assay. Data are shown as mean±SEM for the three replicates. Statistical significance levels are indicated as: *P < 0.05; **P < 0.01 and ***P < 0.001.