Research Paper Volume 9, Issue 4 pp 1233—1247

Aspirin inhibits the SHH/GLI1 signaling pathway and sensitizes malignant glioma cells to temozolomide therapy

Figure 2. Aspirin treatment on the invasive property of glioma cells. (A) U87 and T98G cells migration were analyzed using the scratch-wound assay. (B) U87 and T98G cells invasive ability were analyzed by the transwell assay after 24 h of treatment with indicated concentrations of aspirin. Bars represent 50 μm. (C) Western blot analysis showed that expression of EMT-related markers was altered by aspirin treatment. β-actin was used as an internal control. Data are shown as mean±SEM for the three replicates. Statistical significance levels are indicated as: *P < 0.05; **P < 0.01 and ***P < 0.001.