Figure 2. Aspirin treatment on the invasive property of glioma cells. (A) U87 and T98G cells migration were analyzed using the scratch-wound assay. (B) U87 and T98G cells invasive ability were analyzed by the transwell assay after 24 h of treatment with indicated concentrations of aspirin. Bars represent 50 μm. (C) Western blot analysis showed that expression of EMT-related markers was altered by aspirin treatment. β-actin was used as an internal control. Data are shown as mean±SEM for the three replicates. Statistical significance levels are indicated as: *P < 0.05; **P < 0.01 and ***P < 0.001.