Research Paper Volume 9, Issue 4 pp 1233—1247

Aspirin inhibits the SHH/GLI1 signaling pathway and sensitizes malignant glioma cells to temozolomide therapy

Figure 3. Aspirin induced apoptosis through the internal pathway in glioma cells. (A)The U87 and T98G cells were treated with indicated concentrations of aspirin for 24 h, and the promotion of apoptosis was measured by AnnexinV-FITC/PI double staining. A representative experiment of three replicated assays was shown. (B) Western blot analysis showed Bcl-2, Bax, Caspase 3 and cleaved PARP proteins expression in U87 and T98G cells treated with the indicated concentrations of aspirin for 24h. β-actin was used as an internal control.