Research Paper Volume 9, Issue 4 pp 1233—1247

Aspirin inhibits the SHH/GLI1 signaling pathway and sensitizes malignant glioma cells to temozolomide therapy

Figure 4. Aspirin inhibited SHH/GLI1 signaling pathway. (A) QRT-PCR analysis of SHH, SMO, and GLI1 mRNA from U87 and T98G cells following indicated concentrations of aspirin treatment for 24 h. (B) Western blot analysis showed SHH, SMO, GLI1, Bcl-2 and Foxm1 proteins expression in U87 and T98G cells treated with indicated concentrations of aspirin for 24 h. GAPDH was used as an internal control. (C) Western blot analysis showed SHH, SMO, GLI1, Bcl-2 and Foxm1 proteins expression in U87 and T98G cells treated with the indicated concentrations of aspirin (8 mM for U87, 10 mM for T98G) for different time intervals (8, 16 and 24 h). GAPDH was used as an internal control. (D) U87 cells treated with 8mM aspirin for 24 h were subjected to immunofluorescent assay using anti-GLI1 antibody. Data are shown as mean±SEM for the three replicates. Statistical significance levels are indicated as: *P < 0.05; **P < 0.01 and ***P < 0.001.