Research Paper Volume 9, Issue 4 pp 1269—1279

Protective effect of calretinin on testicular Leydig cells via the inhibition of apoptosis

Figure 3. Effect of calretinin on the apoptosis of Leydig cells. (A) The calretinin expression in the MLTC-1 cells transfected with LV-calb2 was significantly higher (B) The calretinin expression in R2C cells transfected with LV-siRNA-calb2 was significantly lower. (C) The number of apoptotic cells were significantly decreased in MLTC-1 cells with up-regulated calretinin. (D) The apoptotic cells were significantly increased in R2C cells with down-regulated calretinin. (E) In MLTC-1 cells transfected with LV-calb2, the apoptotic index was significantly decreased when compared with the control group. (F) In R2C cells transfected with LV-siRNA-calb2, the apoptotic index was significantly higher when compared with the control group. The vector was used as the negative control(s). *: p<0.05; ***: p<0.001.