Research Paper Volume 9, Issue 4 pp 1293—1306

Functional human GRIN2B promoter polymorphism and variation of mental processing speed in older adults

Figure 1. Binding of recombinant Elk-1 protein to dsDNAs containing the rs3764030 variant A allele from the human GRIN2B gene promoter. Double-stranded DNA targets (Methods) were incubated with1 μg recombinant human Elk-1 protein on in EMSA buffer. DNA-protein complexes were electrophoresed through 6% polyacrylamide gels in HEPES buffer pH 6.3, without polydIdC. The gel was then stained with the SYBR Green to visualize DNA (A) and SYPRO Ruby for protein bound to DNA (B). The stained gel was scanned in G:BOX (Syngene, US) for imaging. Elk-1 bound to DNA is indicated by the arrow.