Figure 2. Concentration dependent response of human GRIN2B promoter in N2a cells carrying either the A or G alleles of rs3764030 to NMDA. Luciferase reporter gene plasmids were constructed in pGL 4.10 (to produce Firefly luciferase, Promega, US). Luciferase reporter plasmids and the pGL4.75 plasmid (to produce Renilla luciferase as a reference) were co-transfected into retinoic acid differentiated N2a cells and assayed for (Methods). After 24 hours of transfection, the cells were incubated for six hours with 0, 30, 50, 70 or 90 μM NMDA for transcription factors binding and then replaced with complete media (with 10% FBS) and cultured for an additional 40-44 hrs. Data are presented as means ± SE.