Research Paper Volume 9, Issue 4 pp 1326—1340

BMP2 promotes proliferation and invasion of nasopharyngeal carcinoma cells via mTORC1 pathway

Figure 2. BMP2 enhances NPC cells growth. (A) The over-expression efficiency of BMP2 in S18 and 6-10B cells were confirmed by Western blot, respectively. And CCK-8 assays showed that BMP2 could significantly promote proliferation of S18-BMP2 and 6-10-BMP2 cells compared with the controls. (B) BMP2 knockdown in 5-8F cell via three specific siRNA was confirmed by Western blot and CCK-8 assays showed that BMP2 knockdown reduced the proliferation of 5-8F cell. (C) In colony formation assays, the number of colonies was significantly higher in the S18-BMP2 and 6-10B-BMP2 cells than the controls.