Research Paper Volume 9, Issue 4 pp 1326—1340

BMP2 promotes proliferation and invasion of nasopharyngeal carcinoma cells via mTORC1 pathway

Figure 3. BMP2 promotes NPC cell migration, invasion and epithelial-mesenchymal transition. (A) Wound-healing assays demonstrated that BMP2 upregulated cells had higher migration rate than control cells. (B) Matrigel invasion chambers were used to evaluate the ability of NPC cell invasion. Upregulation of BMP2 significantly increased the number of invaded cell in S18 and 6-10B compared with the controls. (C) silencing expression of BMP2 in 5-8F much reduced the invaded cell numbers. (D) Western blotting revealed that overexpression of BMP2 in S18 and 6-10B cells decreased the expression of epithelial makers (E-cadherin) and increased the expression of mesenchymal markers (N-cadherin, Vimentin and β-catenin) in comparison with the controls. (E) Western blotting showed that knockdown of BMP2 increased the expression of E-cadherin and decreased the expression of N-cadherin, Vimentin and β-catenin.