**Figure 2.** **LHON disease phenotype in iPSC-derived RGCs can be reversed in corrected cybrid lines.** (**A**) Efficiency in RGC differentiation as assessed by the % of THY1.1 positive cells obtained post MACS sorting for control, LHON and corrected cybrid lines. Data are expressed as mean + SEM of independent samples (n=11 for control, n=8 for LHON and n=8 for corrected cybrid, expressed as pooled data of experimental repeats and biological repeats (3 clones)). (**B**) TUNEL assay revealed increased susceptibility to apoptosis in LHON RGCs and reversal in corrected cybrid lines. Data are expressed as mean of each clone, n = 3 clones, error bars = mean ± SEM. (**C**) Quantification of mitochondrial superoxide levels using MitoSOX in control, LHON and corrected cybrid RGCs. Error bars = ± SEM, n = 3 clones. Statistical significance was established by one way ANOVA followed by Dunnett’s test for multiple comparisons, ** p<0.01, * p<0.05, ns: not significant.