Research Paper Volume 9, Issue 5 pp 1359—1374

Aging-associated oxidative stress inhibits liver progenitor cell activation in mice

Figure 4. Increased neutrophil infiltration in livers of O-CDE mice. (A) mRNA levels of cytokines in livers were measured by Q-PCR. Results are mean ± SEM from three independent experiments (n > 4 mice per group). (B) Quantification of liver-infiltrating leukocytes from Y/O mice with normal/CDE diet by FCM: neutrophils (CD11b+Gr-1high), macrophages (CD11b+Gr-1low), CD4+ T cells (CD3+CD4+) and CD8+ T cells (CD3+CD8+). Results are mean ± SEM from three independent experiments (n > 4 mice per group). (C) mRNA level of MPO in livers was measured by Q-PCR. Results are mean ± SEM from three independent experiments (n > 4 mice per group). (D) MPO(green)/DAPI(blue) staining of liver tissues from Y/O mice with normal/CDE diet. a-d, scale bar = 100 μm; e, scale bar = 20 μm. *P < 0.05, **P < 0.01.