Research Paper Volume 9, Issue 5 pp 1404—1413

Diffuse colonies of human skin fibroblasts in relation to cellular senescence and proliferation

Figure 2. The fraction of SA-βgal positive cells in the colonies of various types. Top panel, the bar plot of mean values of 10 donors studied ± SEM. Bottom panel, representative images of the aging cells (cytoplasm colored dark blue) in each type of colonies. Nuclei were counterstained with Hoechst 33342 (bright light blue).