Figure 2. Loss of mbk-1 decreases lifespan of long-lived daf-2 and glp-1 mutant C. elegans. The effect of loss of function mutations in mbk-1 and hpk-1, mbk-1(pk1389) and hpk-1(pk1393), respectively, on lifespan relative to mbk-1(+) and hpk-1(+) animals was examined in different genetic backgrounds. (A) daf-2(-) [daf-2(e1370)] and corresponding daf-2(+) animals were grown continuously at 20 °C. (B) glp-1(-) [glp-1(e2144ts)] and corresponding glp-1(+) animals were grown at 25 °C for the first 24 h of postembryonic development to eliminate germ cells in glp-1(-) strains, and subsequently, were cultured at 20 °C for the remainder of the experiment. See Table 1 for statistical analysis.