Research Paper Volume 9, Issue 5 pp 1414—1432

The protein kinase MBK-1 contributes to lifespan extension in daf-2 mutant and germline-deficient Caenorhabditis elegans

Figure 3. Effect of C. elegans mbk-1 on DAF-16 target gene expression. (A) Loss of mbk-1 decreases expression of a panel of DAF-16 target genes in glp-1(-) [glp-1(e2144ts)], but not in wild-type or daf-2(-) [daf-2(e1370)] animals as determined by qPCR (representative experiment shown, n=2). Error bars indicate standard deviations of three technical replicates. Statistical significance of expression level differences was determined by two-way ANOVA with Bonferroni post tests. (B) Loss of mbk-1 does not decrease daf-16 mRNA levels as determined by qPCR (representative experiment shown, n=2; error bars and statistical analysis as in panel A). (C) Loss of mbk-1 decreases Psod-3::gfp-expression in glp-1(-), and –to a lesser extent- in wild-type background (representative experiment shown, n=3). Error bars indicate standard deviations. Statistical significance of fluorescence intensity differences was determined by two-way ANOVA with Bonferroni post tests. All experiments in (A)-(C) were performed on day-2 adult worms. Images in (C) were taken at 100x magnification.