Research Paper Volume 9, Issue 5 pp 1453—1469

Aged induced pluripotent stem cell (iPSCs) as a new cellular model for studying premature aging

Figure 1. Behaviour of lamin A/C, nucleoskeleton and mitochondria in iPSCs, in pluripotent state and following differentiation and senescence. (A) Lamin A/C and β-III tubulin staining was absent in y-iPSCs of the colony (asterisk), but normally detectable upon differentiation (red nuclei). Lamin A/C strongly increased in aged cells, whereas β-III tubulin was faintly present (right column: high magnification of the inset). (B) Mean fluorescence intensity of lamin A/C immunostained nuclei in colony (clone) and differentiated (diff) cells from y-iPSCs and from a-iPSCs nuclei. Data are presented as the mean ± SEM (*: p < 0,05). (C) RT-PCR analysis of LMNA mRNA expression in y-iPSCs and a-iPSCs. Reduced levels of lamin A/C are associated with the youth and stemness (*: p < 0,05, n=3). (D) Increased nuclear dysmorphisms were found in a-iPSCs. (E) Abnormal accumulation of mitochondria was noticed in association with nucleoskeletal alterations in senescent iPSCs (arrows).