Research Paper Volume 9, Issue 6 pp 1607—1622

Brain ageing changes proteoglycan sulfation, rendering perineuronal nets more inhibitory

Figure 1. Brain immunohistochemistry shows a progressive increase in C4S and a progressive decrease in C6S from 3 to 18 months of age. (A) Immunostaining of the auditory cortex with antibody LY111, which recognizes C4S, at time points 3 months to 18 months. (B) The histogram shows the quantification of the staining intensity of C4S from 3 to 18 months. (C) Immunostaining of the auditory cortex with antibody MC21C, which recognizes C6S, at time points 3 months to 18 months. (D) The histogram shows the quantification of the staining intensity of C6S from 3 to 18 months. Graphs show mean ± s.e.m. *** indicates a significant relationship between staining intensity and time by Kruskal Wallis, p >0.001. 3m, 3-month-old brains; 6m, 6-month-old brains; 12m, 12-month-old brains; 18m, 18-month-old brains; C4S, Chondroitin-4-sulfate; C6S, Chondroitin-6-sulfate. Scale bars: 200 μm.