Figure 4. mRNA levels for the various chondroitin sulfotransferases do not change with ageing. (A) shows typical PCR results for the various enzymes. The age of brain from which the mRNAs were extracted is shown by bars at the bottom. The number of cycles is shown by the number above each gel. (B) Quantification of the mRNA levels for the sulfotransferases at 4 age points. The method of quantification is described in the methods section. There are no changes of mRNA levels with ageing. Each bar represents results from at least 4 assays. Bars are SEM.