Research Paper Volume 9, Issue 8 pp 1885—1897

Epigenetic silencing of miR-338 facilitates glioblastoma progression by de-repressing the pyruvate kinase M2-β-catenin axis

Figure 1. PKM2 correlated with glioma grade progression and promoted glioma cell proliferation and invasion. (A) PKM2 expression and its correlation with glioma grade prognosis in the CGGA and REMBRANDT. (B) IHC staining depicting the difference in PKM2 expression between low-grade and high-grade gliomas. (C) Kaplan-Meier survival curves indicating cumulative survival as a function of time for patients with high versus low PKM2 expression. (D) Representative images of Transwell assays of cells after transfection. Number of invading cells shown as a histogram (P<0.05). (E) Cell viability was examined with a CCK-8 assay at different time intervals after transfection. (F) ATP level assay at different time intervals after transfection (P<0.05).