Research Paper Volume 9, Issue 8 pp 1885—1897

Epigenetic silencing of miR-338 facilitates glioblastoma progression by de-repressing the pyruvate kinase M2-β-catenin axis

Figure 4. MiR-338 reduced the binding between PKM2 and β-catenin to repress β-catenin transcriptional activity. (A) Co-IP assay to investigate the effect of miR-338 on the binding between PKM2 and β-catenin. (B) U87 and SNB19 cells were transiently transfected (24 h) with the pGL4.74 plasmid and co-transfected with pGL4.75. Cells were then treated as indicated for 48 h. Both firefly and Renilla luciferase activities were calculated and recorded as fold-induction (P<0.05, P<0.05). (C) Cell cycle analysis of miR-Scr/miR-338-transfected cells treated with EGF, and overview of the cell cycle. (D) Immunostaining of β-catenin location after miR-Scr/miR-338-transfected cells were treated with EGF.