Figure 5. Histology to demonstrate increased proportion of Lgr5-EGFP IESC in S-phase in old mice. (A) Representative images of crypt sections from young and old Lgr5-EGFP mice stained with EGFP, S-phase marker EdU and nuclear marker DAPI. Closed arrows indicated Lgr5-EGFP IESC in S-phase. (B) Number of total crypt cells incorporating EdU per crypt section. n=5 young and 6 old animals. (C). Number of Lgr5-EGFP IESC incorporating EdU per crypt section. n=5 young and 6 old animals, *p<0.05 Young vs. Old, unpaired t test. Magnification: 40x, Scale bar: 20µm for all images.