Figure 3. Effects of chronic exercise in Middle-Aged obese mice. Experimental design 2 (A). Body weight change, cumulative food intake and average food intake (A-D) (n=10 per group). VO2 (E), CO2 (F) and ambulatory activity (G) (n= 4 per group). All analyses were made in the last day of training. Western blotting of UCP-1 protein level in BAT (H) and normalization of protein level by Tubulin (right) (I), picture of BAT, highlighting the coloration (J) (n= 6 per group). Data are expressed as means ± SEM. *, p<0.05 vs Sedentary group.