Figure 3. TLR-4 mutant old mice are protected from adipose tissue inflammation. Expression of Tlr4 gene products: mRNA (A) and protein (B) levels together with NF-κB activation by using a phospho-NF-κB p65 pSer311 antibody in western blotting analysis. The relative protein density is expressed in (C). (D) Expression profile of pro-inflammatory cytokine genes (Il-6, Mcp1 and Tnf-a) in the adipose tissue of young or old of both WT and TLR4 deficient mice. (E) The protein product of IL-6 and MCP1 in the SVF lysates derived from the adipose tissues of young WT and old WT or old TLR4 deficient mice. Data represented in bar diagrams are mean + SD value of relative mRNA expression from three independent experiments where total RNA was extracted from gonadal fat pads of young (n=5) and old (n=5) mice. Data presented here are representative images of three independent experiments. The significance levels *p<0.05, **p<0.001 were analyzed by unpaired Student’s t-test using means and SDs.