Figure 5. Reduced expression level of senescence associated genes p16 and p21 in the adipose tissue of TLR4 deficient old mice. Relative mRNA expression of senescence associated genes (A) and their protein products (B) in adipose tissue of WT and TLR4 deficient old mice (20 m). The relative density of protein bands of p16 and p21 are expressed in bar diagram (B, right). Data represented in bar diagrams are mean + SD value of three independent experiments. Significance of difference between means was determined by Student’s t-test and indicated by * p<0.05 and **p<0.01.