Figure 4. The effects of PKC isoform inhibition in RPE cell multinucleation. (A) control RPE cells. (B) oxPOS treated RPE cells. (C) PKCζ inhibited + oxPOS treated RPE cells. (D) PKCι inhibited + oxPOS treated RPE cells. (E) PKC δ/ε inhibited + oxPOS treated RPE cells. Arrows indicate multinucleate RPE cells. (F) histogram showing the percentage of binucleate/multinucleate RPE cells following different treatments *, P < 0.05, ***, P < 0.001 compared to oxPOS treated group. One-way ANOVA followed by Tukey’s multiple comparison test. 50 cells were counted from three wells for each group.