Research Paper Volume 9, Issue 11 pp 2302—2315

Selenium preserves keratinocyte stemness and delays senescence by maintaining epidermal adhesion

Figure 1. Influence of selenium on cell viability and proliferation. Effects on cell viability after application of a range of NaSe concentrations during 72 h for (A) fibroblasts and (B) keratinocytes. Viability is calculated as the “(mean of treated cells–mean of blank) / (mean of untreated cells–mean of blank)” ±SD (n=3 donors and n=12 measurements per conditions). The stars illustrate statistical differences between 100% and mean of experimental points. Effects of 3 Selenium concentrations on clonogenic potential of keratinocytes from 3 donors after 12 days of supplementation. (C) Representative aspect of the colonies at different concentrations versus the control, (n=3; scale bar=1cm) and (D) CFE ratio are calculated to avoid inter-individual heterogeneity of CFE. *p<0.05; **p<0.01; ***p<0.001.