Figure 2. MiR-30a expression level during aging. The expression levels of miR-30a-3p and miR-30a-5p were evaluated by QPCR in (A) keratinocytes from infant and adult male donors and (B) skin equivalent (SE) model mimicking aging with a long time of culture: MiR-30a expression was monitored along the aging process from day 35 (D35) to day 100 (D100) of culture. For QPCR: results are mean +/- SD from three independent samples. *P< 0,05, **P<0,01. (C) The expression levels and the localization of miR-30a-3p and miR-30a-5p were evaluated by in situ hybridization in young and aged skin. Scale bar = 50 μm. Representative pictures were shown.