Research Paper Volume 9, Issue 11 pp 2376—2396

An expression screen for aged-dependent microRNAs identifies miR-30a as a key regulator of aging features in human epidermis

Figure 4. Identification of potential miR-30a targets in human epidermis. (A) A list of genes potentially targeted by miR-30a according to transcriptome data available in the GEO database. The number of conserved miR-30a MRE is indicated for each gene. The potential expression of these genes in human epidermis (according to the Human Protein Atlas database) is indicated. Five genes exhibiting a least one miR-30a conserved MRE and expressed in human epidermis, were analyzed by QPCR in RNA samples from skin equivalent (SE) model mimicking aging. The expression profile is indicated for these 5 genes. (B) The expression levels of AVEN, IDH1 and LOX were evaluated by QPCR in RNA samples from SEs mimicking aging with a long time of culture from day 35 (D35) to day 100 (D100). Results are mean +/- SD from three independent samples. *P< 0,05, ****P<0,0001. (C) Expression of AVEN, IDH1 or LOX in young or old human skin sections assessed by immunofluorescence for AVEN and IDH1 and by immunohistochemistry for LOX. (D) Quantification of the AVEN, IDH1 and LOX labeled area in the epidermis from young or old human skin sections. Results are mean +/- SD from three independent samples. ***P<0,001, **P<0,01.