Research Paper Volume 9, Issue 11 pp 2397—2410

Residual γH2AX foci induced by low dose x-ray radiation in bone marrow mesenchymal stem cells do not cause accelerated senescence in the progeny of irradiated cells


Figure 1. Immunocytochemical analysis of the γH2AX foci in the MSCs irradiated with low (80 mGy) vs. intermediate (1000 mGy) dose of X-ray radiation. (A) Representative microphotographs of the immunofluorescently stained irradiated MSCs with γH2AX foci showed in red. DAPI nuclear counterstaining is shown in blue. (B) Comparative changes in the γH2AX foci number in cells exposed to the low or intermediate dose of X-ray radiation. (C) Levels of γН2АХ foci expressed relative to maximum levels at 0.5 h after irradiation demonstrating the difference in the rate of foci clearance after low vs. intermediate doses. Mean values derived from at least three independent experiments are shown. Error bars represent standard error (SE).