Figure 2. Functional differentiation profile of CD4+ and CD8+ T-cells in patients with polypoidal choroidal vasculopathy (PCV), patients with neovascular age-related macular degeneration (nAMD), and healthy controls. Cells were defined as naïve T-cells CD45ra+CD45ro-CCR7+, central memory T-cells (CM) CD45ra-CD45ro+CCR7+, effector memory T-cells (EM) CD45ra-CD45ro+CCR7-, and effector memory CD45ra+ T-cells (EMRA) CD45ra+CD45ro+CCR7-. These subsets were separately identified in CD4+ T-cells (A) and CD8+ T-cells (B). (C) We found no significant differences between patients with PCV, patients with nAMD, and healthy controls (P > 0.05 for all comparisons using Kruskal-Wallis test). (D) Age-related changes in the ratio of memory to naïve T-cells indicated no age-related change in CD4+ T-cells, but a clear age-related increase in CD8+ T-cells that appeared stronger in patients with nAMD.