Figure 3. T-cell differentiation measured as CD27 and CD28 downregulation in patients with polypoidal choroidal vasculopathy (PCV), patients with neovascular age-related macular degeneration (nAMD), and healthy controls. CD4+ T-cells lose CD27 first and CD28 later, whereas the opposite is the case for CD8+ T-cells which lose CD28 first and CD27 later. (A) Identification of CD27-, CD28-, and CD28- in CD27- CD4+ T-cells. (B) Identification of CD28-, CD27-, and CD27- in CD28- CD8+ T-cells. (C) We only found a difference in CD27- in CD28- CD8+ T-cells (signified with *, P = 0.040, Kruskal-Wallis test), where patients with nAMD which had significantly higher proportion compared to the other groups. (D) Age-related changes in CD27 and CD28 loss were comparable between the groups. CD4+CD28- and CD4+CD27-CD28- were not included due to numbers close to the extremes yielding unstable correlation analyses.