Research Paper Volume 9, Issue 12 pp 2480—2488

Transfer of autologous mitochondria from adipose tissue-derived stem cells rescues oocyte quality and infertility in aged mice

Figure 3. Improved oocyte quality of aged mice by autologous ADSC mitochondria supplement. The GV oocytes were collected from each mouse and microinjected with mitochondria extracted from its autologous ADSCs in HTF(Mito), and the GV oocytes in control group were microinjected with HTF(Ctrl). Then the oocytes were cultured to mature in vitro, and the matured MII oocytes were checked for mtDNA numbers, spindle/chromosome morphology and karyotype. (A) Increased mtDNA numbers in MII oocytes of aged mice after mitochondria supplement. The mtDNA number increased dramatically after autologous ADSC mitochondria supplement compared to the control [(12.47±4.16) X 104 VS (8.38±1.99) X 104)]. The numbers of MII oocytes measured were indicated. (B) Increased normal morphology of spindle and chromosomes in MII oocytes of aged mice after mitochondria supplement. Bar=50μm. (C) Decreased aneuploidy in MII oocytes of aged mice after mitochondria supplement. Bar=50μm.